Journal: iScience

Article Title: Robust small molecule-aided cardiac reprogramming systems selective to cardiac fibroblasts

doi: 10.1016/j.isci.2023.108466

Figure Lengend Snippet:

Article Snippet: Inserts and linearized vector were assembled using a pEASY-Uni Seamless Cloning and Assembly Kit (TransGen, Cat#CU101). tetO-hGATA4 (addgene, #46030), tetO-hMEF2C (addgene, #46031), and tetO-hTBX5 (addgene, #46032) were purchased from addgene.

Techniques: Recombinant, Cloning, Plasmid Preparation, cDNA Synthesis, Software, Knock-Out

Journal: World Journal of Stem Cells

Article Title: Overexpression of GATA binding protein 4 and myocyte enhancer factor 2C induces differentiation of mesenchymal stem cells into cardiac-like cells

doi: 10.4252/wjsc.v14.i9.700

Figure Lengend Snippet: Primer sequence and annealing temperature of cardiac genes

Article Snippet: GATA-4 and MEF2C plasmids were purchased from Addgene (plasmid No. 46030 and No. 46031, respectively).

Techniques: Sequencing

Journal: World Journal of Stem Cells

Article Title: Overexpression of GATA binding protein 4 and myocyte enhancer factor 2C induces differentiation of mesenchymal stem cells into cardiac-like cells

doi: 10.4252/wjsc.v14.i9.700

Figure Lengend Snippet: Gene expression analysis of GATA binding protein 4 and myocyte enhancer factor 2C transfected human umbilical cord mesenchymal stem cells. Semiquantitative real-time polymerase chain reaction (RT-PCR) analysis was performed to show the gene expression levels of GATA binding protein 4 and myocyte enhancer factor 2C transfected mesenchymal stem cells, separately and in combination, in comparison to the control. Results are expressed as the mean ± SE ( n = 3). Differences between groups are considered statistically significant where b P < 0.01 and c P < 0.001. GATA-4: GATA binding protein 4; MEF2C: Myocyte enhancer factor 2C.

Article Snippet: GATA-4 and MEF2C plasmids were purchased from Addgene (plasmid No. 46030 and No. 46031, respectively).

Techniques: Gene Expression, Binding Assay, Transfection, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Comparison, Control

Journal: World Journal of Stem Cells

Article Title: Overexpression of GATA binding protein 4 and myocyte enhancer factor 2C induces differentiation of mesenchymal stem cells into cardiac-like cells

doi: 10.4252/wjsc.v14.i9.700

Figure Lengend Snippet: Morphological changes and cardiac-specific gene expression in transfected human umbilical cord mesenchymal stem cells. A: Images showing human umbilical cord mesenchymal stem cells trasnfected with (b) GATA binding protein 4 (GATA-4), (c) myocyte enhancer factor 2C (MEF2C), and (d) GATA-4 + MEF2C, and (a) the corresponding untreated control. All images were captured at day 14 under a phase contrast microscope (scale bar: 100 μm); B: Bar diagrams showing fold change analysis of cardiac gene expression by semiquantitative real-time polymerase chain reaction (RT-PCR) in the transfected cells in comparison to the control cells after 14 d of culture. Results are expressed as the mean ± SE ( n = 3). Differences between groups are considered statistically significant where a P < 0.05, b P < 0.01, and c P < 0.001. GATA-4: GATA binding protein 4; MEF2C: Myocyte enhancer factor 2C; MHC: Myosin heavy chain; NKX2.5: NK2 homeobox 5.

Article Snippet: GATA-4 and MEF2C plasmids were purchased from Addgene (plasmid No. 46030 and No. 46031, respectively).

Techniques: Gene Expression, Transfection, Binding Assay, Control, Microscopy, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Comparison

Journal: World Journal of Stem Cells

Article Title: Overexpression of GATA binding protein 4 and myocyte enhancer factor 2C induces differentiation of mesenchymal stem cells into cardiac-like cells

doi: 10.4252/wjsc.v14.i9.700

Figure Lengend Snippet: Cardiac-specific protein expression in transfected human umbilical cord mesenchymal stem cells. A: Fluorescence images showing human umbilical cord mesenchymal stem cells (hUC-MSCs) transfected with GATA binding protein 4 (GATA-4) and myocyte enhancer factor 2C (MEF2C), separately and in combination, in comparison to the control cells (scale bar: 50 μm); B: Bar diagrams showing quantification of positive cells using ImageJ software. Also shown is the comparison between the individual and combination groups. Results are expressed as the mean ± SE ( n = 5). Differences between groups are considered statistically significant where a P < 0.05 and c P < 0.001. GATA-4: GATA binding protein 4; MEF2C: Myocyte enhancer factor 2C; CTnT: Cardiac troponin T; NKX2.5: NK2 homeobox 5; NS: Not significant.

Article Snippet: GATA-4 and MEF2C plasmids were purchased from Addgene (plasmid No. 46030 and No. 46031, respectively).

Techniques: Expressing, Transfection, Fluorescence, Binding Assay, Comparison, Control, Software